The first thing to ask is, do you really need to construct your own cDNA library, or can you get one from someone else? If you can get one from someone else, this can put you weeks or months ahead in terms of time. If you decide you really need your own, then make sure you carefully decide on the source of the tissue or cells, making sure of their purity, and make sure you use good technique during mRNA isolation.
Good luck!
Before constructing c-dna library, what is the two things that should be taken into our mind.?
well u have to keep in mind if ure synthesizing the cDNA from the 3'-5' end or frm the 5'-3' end... u have to keep in mind abt th purity of the mRNA u have taken.. and also u will have a large number of enzymes tht u can choose to hlp in ure cDNA synthesis.. u shld be able to decide which is the best suited enzymes to suit ure criteria..
Subscribe to:
Post Comments (Atom)
No comments:
Post a Comment